- How do you calculate response factor?
- How do you calculate RRT and RRF?
- Why is the detector response factor determined?
- What causes RSD failure in HPLC?
- Why pH is important in HPLC?
- How many types of pumps are there in HPLC?
- How is peak purity calculated?
- What is RT in HPLC?
- How do you calculate impurities?
- What is difference between RF and RRF?
- What is difference between potency and purity?
- What is difference between assay and purity?
- How is purity calculated from HPLC?
- What is RRT and RRF in HPLC?
- What is the relative response factor?
How do you calculate response factor?
Response Factor = Peak Area / Concentration It is important to remember that variations in a gas chromatography (GC) system and analysis methodology can be the cause of a deviation in the response factor..
How do you calculate RRT and RRF?
Divide the RT of the peak of interest by the RT of the main peak to find the RRT of the peak of interest. In our case, this would be 1.5 minutes/3 minutes, or 0.5.
Why is the detector response factor determined?
The response factor is a correction factor allowing the calculation of the true value of an analyte’s concentration when using internal standard calibration. The response factor represents differences in response between the analyte(s) and the internal standard for a particular detector.
What causes RSD failure in HPLC?
The most common cause of peak retention time drift in one direction is poorly prepared or mixed solvents or a system leak.
Why pH is important in HPLC?
When samples contain ionisable compounds, mobile phase pH can be one of the most important variables in the control of retention in a reversed‑phase HPLC (RP-HPLC) separation. … Since most compounds analysed by RP-HPLC contain one or more acidic or basic functional groups, most mobile phases require pH control.
How many types of pumps are there in HPLC?
3There are 3 main types of HPLC pumps: 1. reciprocating pump – 90% usage. The solvent is drawn into a small chamber (when the solvent check valve is open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor-driven piston (Fig 28-6 or R5 Fig 6.9).
How is peak purity calculated?
All spectra in the peak are compared to the apex spectrum, which is used as an internal reference point. A photodiode array detector is used to determine peak “purity” by determining spectral homogeneity across the peak. This is sometimes referred to as peak homogeneity.
What is RT in HPLC?
Retention time (RT) is a measure of the time taken for a solute to pass through a chromatography column. It is calculated as the time from injection to detection. The RT for a compound is not fixed as many factors can influence it even if the same GC and column are used.
How do you calculate impurities?
Calculation of percentage contents: – correction factors: multiply the peak areas of the following impurities by the corresponding correction factor: impurity A = 0.7; impurity F = 0.3; – for each impurity, use the concentration of diclofenac in reference solution (a).
What is difference between RF and RRF?
RRF is used to correct the difference in detector response of impurities with analyte peak. RRF is established by slope method with linear range of solutions. … As per United States Pharmacopoeia (USP) The Relative response factor, is the ratio of the responses of equal amounts of the Impurities and the drug substance1.
What is difference between potency and purity?
potency often means the amount of the desired compound in the sample: when you weigh 100 mg of a standard with 90% pontency, there’s only 90 mg of the targeted compound in there. in most cases, purity = chemical purity. that is, percentage of the target compound as determined by the primary method.
What is difference between assay and purity?
The main difference between assay and purity is that an assay is the determination of one of the main component in a sample whereas purity is the determination of impurities in a sample. … Assay and purity are two types of measurements used to determine the components of a sample.
How is purity calculated from HPLC?
High Performance Liquid Chromatography (HPLC) We use this method to determine the purity of our products. The ratio of the desired product to that of the combined impurities is expressed as a % purity. We will typically state that a product is say >99% (by HPLC).
What is RRT and RRF in HPLC?
The relative retention time (RRT) is the comparison of the RT of one compound to another. Relative Response Factor (RRF) is an analytical parameter used in chromatographic procedures to control impurities/degradants in drug substance and drug product.
What is the relative response factor?
Relative response factor is the ratio of the response of the impurity and the active pharmaceutical ingredient (API) under the identical chromatographic conditions (chromatographic column, temperature, mobile phase, flow rate etc).